The Study on the mtDNA Mutation in Papillary Thyroid Carcinoma
ZHOU Chen, JIANG Zhiying, LIU Yihong, BAI Yidong*
This study explored the correlation between mtDNA (mitochondrial DNA) mutation and thyroid carcinoma, and evaluated the diagnostic value of mtDNA copy number for thyroid carcinoma. Analyzed therelationship between mutation rate and clinical data after mtDNA sequencing and haplotype typing in patients with nodular goiter, follicular thyroid adenoma and papilla thyroid carcinoma. Finally, the mtDNA copy number in tissue and blood samples of three groups was measured by fluorescence quantitative PCR. The results showed that the mtDNA mutation rates in complex I subunit coding region and tRNA coding region of patients with papillary thyroid carcinoma were significantly higher than those in nodular goiter, and mtDNA haplotype M had lower lymph node metastasis than haplotype N in patients with papilla thyroid carcinoma. The results of fluorescence quantitative PCR showed that the mtDNA copy number in the tissue of patients with thyroid adenoma and thyroid carcinoma was significantly higher than that in nodular goiter, but both of them had lower mtDNA copy number in the blood samples than that in nodular goiter. These results suggest that the mtDNA copy number changes and complex I subunit coding region mutations may be used as biological indexes for the diagnosis of thyroid carcinoma, and the mtDNA haplotype N may be used as an early warning index for malignant changes of papillary thyroid carcinoma.
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