MiR-15b Induces Acute Promyelocytic Leukemia Cells Differentiation and Inhibits Proliferation

Yuan Zhen^1,2, Liu Beizhong^1,2, Liu Dongdong^1,2, Yao Juanjuan¹, Zhong Pengqiang¹, Liu Junmei¹, Yao Shifei¹,

Zhao Yi¹, Chen Min¹, Liu Lu^1,2, Li Lianwen¹, Zhong Liang²*

(¹Central Laboratory, Yongchuan Hospital Affi liated to Chongqing Medical University, Chongqing 402160, China; ²Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, Chongqing Medical University, Chongqing 400016, China)

Abstract:

This study aimed to investigate the effects of miR-15b on cell differentiation and proliferation in acute promyelocytic leukemia cell line NB4 cells. In this study, the expression of miR-15b was detected by qRT-PCR, the expression of myeloid differentiation marker CD11b was detected by flow cytometry and cell proliferation was examined by CCK-8 assay. Luciferase reporter assays and Western blot were used to identify miR-15b regulating its target genes. These results indicted that miR-15b gradually increased upon ATRA treatment in a time dependent manner. MiR-15b mimic treatment significantly increased the percentage of CD11b-positive cells and inhibited cell proliferation. MiR-15b inhibitor significantly decreased the percentage of CD11b-positive cells. Luciferase reporter assays and Western blot demonstrated miR-15b could regulate the level of CCNE1. These results suggest that miR-15b inhibits cell proliferation and promotes cell differentiation by inhibiting the expression of CCNE1.

CSTR: 32200.14.cjcb.2019.05.0016