Endogenous SDF-1 from the hUC-MSCs Promotes Improvement of Nerve Function in the HIBD Rats

Yang Ting, Gu Jialu, Zhou Xiaoqin, Gu Yan, He Mulan, Li Tingyu, Chen Jie*

(Children’s Research Center, Children’s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Child’s Nutrtion and Health, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing 400014, China)

Abstract:

The paper mainly studies the role and mechanism of human umbilical cord mesenchymal stem cells (hUC-MSCs) in promoting neurological repair of hypoxic-ischemic brain damage (HIBD). After transplantation of hUC-MSCs, HIBD rats were observed for behavior, histopathological changes of hippocampus were observed by hematoxylin-eosin staining, the supernatant in co-culture system of hUC-MSCs and oxygen glucose deprivation (OGD) neural stem cells was detected by Western blot, the expression of SDF-1a, CXCR4 and PI3K/AKT in hippocampus tissue of HIBD rats were detected, and the proliferation of stem cells was measured by CCK-8. The results showed that after transplantation of hUC-MSCs, the learning and memory function and hippocampal pathological changes of HIBD rats were improved, the expression of Nestin in hippocampus and the number of regenerated neural stem cells in dentate gyrus of hippocampus were increased, and the expressions of hSDF-1a, SDF-1a, CXCR4, PI3K, AKT and p-AKT in hippocampus were up-regulated. hUC-MSCs promote the proliferation and secretion of hSDF-1 alpha in nerve stem cells injured by OGD, and up-regulate the expression of PI3K and p-AKT.

CSTR: 32200.14.cjcb.2019.05.0013