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Influence of Placenta Mesenchymal Stem Cells on Proliferation and Migration of Keratinocytes under High Glucose Conditions
Liu Shudan1, Ma Huiming2, Liang Xueyun1, Ma Xiaona1, Yang Tingting1, Chen Dongmei1*
1Institute of Human Stem Cells, General Hospital of Ningxia Medical University, Yinchuan 750004, China; 2Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan 750004, China
Abstract: The aim of this study was to investigate whether MSCs from the human placenta could be an effective therapeutic candidate to promote the proliferation and migration of keratinocytes in yperglycemic environment. We adopted a strategy of treating hKCs with high D-glucose， applying PMSCs in a glucose-induced cell injury model， which is of great significance to elucidate the role of PMSCs in promoting the wound healing such as diabetic foot. In this study， the keratinocytes were obtained and cultured in vitro with various different D-glucose concentrations (12.5 mmol/L， 25 mmol/L， 50 mmol/L， and 100 mmol/L) cultured under routine condition (serum-free medium) for 3 days， the doubling time， cell migration and cell apoptosis assays were performed on human keratinocytes. In all experimental groups the keratinocytes were further cultured with human placenta mesenchymal stem cells. Cell proliferation， apoptosis， and migration were assessed and the expression of vimentin was detected using immunofluorescence and immunoblotting assay thereafter. Keratinocytes presented showed a low proliferation rate and migration areas were sparse in the simulation of hyperglycemia environment (50 mmol/L， and 100 mmol/L Dglucose). Compared with the control group， PMSCs co-clutured group presented more rapid cell growth， higher proliferation rate， and lower apoptosis percentage under high-glucose conditions. In the PMSCs co-clutured group， larger areas were overlaid; and the expression of vimentin was significantly enhanced. These findings suggested that PMSCs could effectively promote keratinocytes survival and migration under hyperglycemia conditions in vitro. It might function via upregulating the expression of vimentin. These results indicated that PMSCs could not only inhibit the apoptosis of human keratinocytes caused by high glucose， but also promote its proliferation and migration under hyperglycemia condition. These advances provided new evidence for mesenchymal stem cells to promote skin wound healing in diabetic patients.
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