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Expression of SK4 in Papillary Thyroid Carcinoma (PTC) and Its Roles in PTC Development
Chen Guanhua1,2, Li Yibo2,3, Wu Huihui2,3, Yu Shengjian1,2, Ma Huailu2,3, Liang Yong1,2*
1The First Clinical College of Wenzhou Medical University, Wenzhou 325000, China; 2School of Medicine, Taizhou University, Taizhou 318000, China; 3Hebei North University, Zhangjiakou 075000, China
Abstract: We detected the mRNA and protein levels of SK4 with Real-time PCR， Western blot and immunohistochemistry in PTC tissues and cell line， and explore the relationship between SK4 and the clinicopathological characteristics of PTC. Simultaneously， after down-regulation of SK4 by its specific inhibitor (TRAM-34)， we use CCK-8， plate cloning assay and Transwell assay to research the effects of SK4 on proliferation， colony formation and migration of PTC cell line CGTHW-3 respectively and the possible molecular mechanisms. The results showed that the mRNA and protein expression of SK4 in PTC tissues were significantly higher than that those in paracancerous tissue (P<0.01). The immunohistochemistry results indicated that the positive rate of SK4 was obviously higher than that of normal thyroid tissues (P<0.01). Nevertheless， there was no significant relationship between the positive rate of SK4 protein expression and clinicopathological features such as gender， age， tumor size， and lymph node metastasis in PTC patients (P>0.05). TRAM-34 can inhibit the proliferation， colony formation and migration of PTC cells by blocking SK4. The above results suggest that SK4 channels are highly expressed in PTC tissues and cells and participate in regulating the proliferation and migration of PTC cells， which may be related to the molecular mechanism of PTC. SK4 may become a new therapeutic target for PTC.
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