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Effects of Astragalus Polysaccharides on Expression of IL-6R， TNFR and Tumor Related Genes of Bone Marrow Mesenchymal Stem Cells in Inflammatory Microenvironment
Luo Yali1,2,3, Zhang Liying2,3, Li Chenghao2, Zhang Yanhui2, Liu Yongqi2*, An Fangyu2,3, Feng Caiqin2, Li Yan2
1Department of Pathology, Basic Medical College, Gansu University of Chinese Medicine, Lanzhou 730000, China; 2Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research, Gansu University of Chinese Medicine, Lanzhou 730000, China; 3Key Laboratory of Dunhuang Medicine, Ministry of Education, Gansu University of Chinese Medicine, Lanzhou 730000, China
Abstract: The purpose of this study is to observe the expression of IL-6R and TNFR of bone marrow mesenchymal stem cells in inflammatory microenvironment induced by IL-6 and TNF-α， and to investigate the effect of astragalus polysaccharide on IL-6R and TNFR expression in order to explore the possible mechanism of IL-6 and TNF-α on inflammatory injury of BMSCs. The inflammatory microenvironment was established by using 100 ng/mL IL-6 and 50 ng/mL TNF-α. BMSCs were divided into control group， model group (D group) and 50 μg/mL APS intervention group (H group). The expression level of sIL-6R in the supernatant was determined by ELISA. The levels of IL-6R， GP-130， TNFR I and TNFR II were detected by immunofluorescence methods. Western blot was used to detect the expression level of P53， PTEN and Ras and C-myc. Results showed that compared with the control group， the content of sIL-6R in the supernatant decreased， and the levels of IL-6R， GP 130， TNFR I and TNFR II increased in model group. Compared with the model group， the interposition of APS increased the content of sIL-6R and decreased the expression of IL-6R， GP 130， TNFR I and TNFR II. Compared with the control group， the expression of Ras， C-myc protein increased and that of P53 and PTEN decreased in model group. After the intervention of APS， the expression of Ras， C-myc protein decreased， while P53 and PTEN protein increased. Collectively， the present study demonstrates that APS could not only decrease the influence of BMSCs on IL-6R and TNFR in inflammatory microenvironment， but also maintain the expression level of tumor related genes stability.
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