Saikosaponin from Bulperum Chinese DC Represses Proliferation and Migration of Gastric Cancer Cell MGC80-3 via Akt/NF-κB Pathway

Abstract: The inhibitory effect of saikosaponin from Bulperum Chinese DC on gastric cancer line MGC80-3 was accessed in this study. And then， the potential molecular mechanism was investigated. Saikosaponin was extracted from Bulperum Chinese DC using the reported method. Effect of saikosaponin on MGC80-3 cell morphology was accessed with the light microscope. Effect of saikosaponin on cell proliferation was determined by MTT method and colony formation assay. Migration capability of MGC80-3 was detected by the cell adhesive assay and wound-healing assay. Western blot was used to access the potential molecular mechanism. Saikosaponin from Bulperum Chinese DC repressed the capability of MGC80-3 cell proliferation and migration in a dose- and time-dependent manner. Saikosaponin significantly reduced the expression level of NF-κB p65， but not affect that of Akt and IKK β. Besides， phosphorylation of Akt and IKK β was decreased in cells treated by saikosaponin. It is probable that saikosaponin from Bulperum Chinese DC repressed MGC80-3 cell via Akt/NF-κB pathway， and showed potential value in the development of anti-gastric cancer drugs.
    

CSTR: 32200.14.cjcb.2018.10.0013