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Effects of miRNA-155 on the Cytoskeletal Pathways in REV and ALV-J Co-Infection
Li Ling1, Zhuang Pingping1, Wang Xiaoman1, Zhou Defang1, Xue Jingwen1, Cheng Ziqiang1,2, Wang Guihua1,2*
1College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China; 2Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian 271018, China
Abstract: This study was aimed to investigate the effects of miRNA-155 expression on actin cytoskeletal pathways in ALV-J and REV co-infection. The exosomes from ALV-J， REV and ALV-J+REV infected DF-1 cells were used to quantity the differentially expressed proteins and miRNAs by proteomic and transcriptome detection technology. Then， the common changed factor and their participation in the signal pathway in co-infected group compared with single infected group were screened. The results showed that ALV-J and REV had synergistic effects on each other， and REV played a leading role in synergism on ALV-J. RhoJ， NACKP1 and ARPC5， and the miRNA-155 target protein ITG were involved in the actin cytoskeleton pathway. After transfection of miRNA-155 inhibitors or mimics， RT-PCR was used to detect the effects of miRNA-155 expression on the virus replication， and the expression of ITG， RhoJ， NCKAP1 and ARPC5 as well. The results demonstrated that the virus replication and the expression of RhoJ and NCKAP1 were promoted， whereas， ITG and ARPC5 expression was decreased， when the miRNA-155 expression was increased. Inhibition of miRNA-155 expression inhibited virus replication， blocked the expression of RhoJ and NCKAP1， and increased the expression of ITG and ARPC5. These changes were more significant in the co-infected group， which was consistent with the previous results that the changes of associated proteins induced by virus infection. Our results suggested that the synergistic effect of ALV-J and REV co-infection might be associated with the miRNA-155 regulation the 4 proteins in actin cytoskeleton pathway. The miRNA-155 is a key factor regulating the synergistic effects of the two viruses.
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