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Androgen Regulates Palmitoylation of Ribosomal Proteins and Aminoacyl-tRNA Synthetases in the Prostate Cancer-Derived LNCaP Cells
Li Wenqing1, Zhang Wei2, Cui Luwei3, Diao Tongxiang3, Zhang Dalei3, Zhang Yaqun3, Xiao Fei1, Liu Ming3*
1The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing 100730, China; 2Department of Pathology, Beijing Hospital, Beijing 100730, China; 3Department of Urology, Beijing Hospital, Beijing 100730, China
Abstract: Firstly， the LNCaP cells were treated with androgen (R1881) or DMSO and meanwhile the cells were metabolically labeled with Alk-C16， an alkyne analogue of palmitic acid. Secondly， the proteins conjugated with Alk-C16 were enriched in azide agarose beads based on the alkyne-azide reaction (click chemistry). At last， the conjugated proteins were digested for mass spectrum (MS) and label-free quantitation. By comparing the protein palmitoylation levels between androgen-treated LNCaP cells and the non-treated， the proteins of which the palmitoylation levels were induced by androgen were screened and identified. The results demonstrated that androgen treatment significantly increased the palmitoylation levels of ribosomal protein RPL12， RPS4X and glutamylprolyl- tRNA synthetase (EPRS). In addition， androgen treatment also increased the palmitoylation levels of glyclytRNA synthetase (GARS) in cell supernatant. The regulation of the palmitoylation for RPL12， RPS4X and EPRS might provide new directions for the therapy of prostate cancer. Moreover， the high palmitoylation level of RPL12， RPS4X and EPRS induced by androgen may be tumor biomarkers. The high palmitoylation level of GARS induced by androgen in supernatant might be a biomarker for tumor early screening.
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