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The Role of Pin1 in the Atherosclerosis and Senescence of Vascular Smooth Muscle Cell
Liang Wei1*, Lü Lei2, Wang Peng1, Zhang Xue1, Yuan Kai1, Li Maoran1, Zhang Jiwei1, Meng Qiurong1
1Department of Vascular Surgery, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201112, China; 2Department of Vascular Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
Abstract: The senescence of vascular smooth muscle cell (VSMC) is greatly related to the pathologic progression of atherosclerosis. However， little is known about the mechanisms behind. Peptidyl-proplyl isomerase (Pin1) is prevalently overexpressed in human cancers. It is implicated to regulate the growth and apoptosis of cell. Thus far， no role of Pin1 has been described in modulating the senescence of VSMC. The method of Western blot was used to confirm the protein level of Pin1 decreased in human atherosclerotic VSMC (P<0.05). The expressions of proteins such as p53， p21， growth arrest and DNA-damage-inducible protein 45-alpha (Gadd45a)， p65 were significantly up-regulated (P<0.05). Meanwhile， the method of β-galactosidase staining was used to confirm that the senescence of atherosclerotic VSMC was more serious than normal VSMC. Adenoviral-mediating Pin1 overexpression led to down-regulation of 53， p21， Gadd45a， p65. These findings indicated that the senescence of VSMC mediated by Pin1 was an integrated response to diverse signals. Our study may provide a novel target for regulation and control of atherosclerosis.
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