Effects on Mucous Cells Distribution in Digestive Tract of Scortum barcoo

Xie Mujiao1, Zhong Jinxiang2, Xie Xiaoyong1*, Zhu Changbo1, Li Junwei1, Wang Jun1, Ye Guoling1,3, Bao Yuyuan1,4

1Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2Guangdong Provincial Ocean and Fishery Technical Extension Center, Guangzhou 510222, China; 3College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China; 4College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

Abstract: We evaluated the effects of starvation on mucous cells features in digestive tract of Scortum barcoo. Fishes were deprived of feed for twenty days， sampled from first day (S0)， tenth day (S10) and twentieth day (S20) and the analysis of mucous cells in digestive tract were examined using AB-PAS (Alician blue and periodic acid Schiff reagent， Alician blue at pH2.5) staining technology. No death was found after the starvation. Type II， III， IV mucous cells were found in esophagus mucosa and starvation had no effect on the type of mucous cells， while impacted the density deeply， statistic showed that mucous cells densities in S0， S10， S20 were (2 839.13±261.52)， (1 546.00±70.02)， (3 095.00±162.04) cells/mm2 respectively. In group S0 and S10， type I and II mucous cells were found in pyloric ceeca， density increased from (215.22±21.52) cells/mm2 to (466.31±63.05) cells/ mm2， but decreased as (369.46±43.49) cells/mm2 with only type II mucous cells in group S20. Type II mucous cells in intestine were increasing obviously after starvation， especially in hindgut， where the densities were (1 683.50±219.64)， (2 068.71±65.38)， (4 622.50±85.60) cells/mm2 in group S0， S10， S20， respectively. The different responds of mucous cells distribution in different localization of digestive tract showed the difference structure and function in different position. With type II mucous cells changing amazingly， we speculated that the secretion of type II mucous cells could be used as a sensitive indicator to reflect starvation stress.

CSTR: 32200.14.cjcb.2018.10.0008