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TML， One Subunit of TPLATE Complex， Regulates Pollen Development in Arabidopsis
Cao Mengxing, Wang Hao, Han Xiao, Gao Xinqi*
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China
Abstract: Clathrin-mediated endocytosis plays roles in the regulation of pollen tube growth; however， its function in pollen development remains to be investigated. TML is one subunit of Arabidopsis TPLATE complex， which is implicated in clathrin-mediated endocytosis. In this study， we created an Arabidopsis mutant of TML， tml- 3， by using CRISPR/Cas9 strategy， in which the pollen showed abnormal morphology and germination. TML was expressed in the pollen from stage 11 and its protein was localized in plasma membrane in pollen from stage 12. The expression of TML was enhanced accompanied with the development of pollen， which was correlated with the ratio of the abortion pollen in the tml-3/+ mutant. Furthermore， we found that the distribution and content of the polysaccharide were changed in the mutant pollen， such as callose， pectin， and cellulose， which suggests that the TML-regulated endocytosis is implicated in Arabidopsis pollen development by regulating polysaccharide accumulation.
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