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The Effects and Mechanism of CCDC3 on Proliferation， Migration and Invation of Human Colon Cancer Cells
Jia Yanmei1#*, Chen Lirong1#, Ni Juhua2
1Laboratory Medicine Department in Fenyang College of Shanxi Medical University, Fenyang 032200, China; 2Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beiing 100191, China
Abstract: We studied the ability of CCDC3 gene on proliferation， migration and invasion of human colon cancer HCT116 cells and its regulatory mechanism in this study. The effect of CCDC3 on the proliferation of human colon cancer HCT116 cells was detected by CCK-8 assay. The effect of CCDC3 on the colony-forming ability of cancer cells was detected by plate clone and soft agarose. The effect of CCDC3 on the migration and invasion of HCT116 cells was detected by Transwell assay. The effect of CCDC3 on the protein level of epithelial-mesenchymal transition in HCT116 cells was detected by Western blot. The effect of CCDC3 on the tumorigenic ability of HCT116 cells was detected by tumor formation assay in vivo. The results showed that CCDC3 played an important role in the regulation of cancer cells growth. Overexpression of CCDC3 significantly promoted the growth of cancer cells， enhanced the colonyforming ability of cancer cells and enhanced the migration， invasion and tumorigenic abilities of cancer cells. After interfering with the expression of this gene， the malignancy of cancer cells weakened. At the same time， we also found that the expression of CCDC3 was associated with epithelial-mesenchymal transition of cancer cell. These findings
suggested that CCDC3 might be a new oncogene that provided clues for finding new therapeutic targets for colon cancer.
suggested that CCDC3 might be a new oncogene that provided clues for finding new therapeutic targets for colon cancer.
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