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Application of Optimized Silver Staining in the Gel Dyeing of Myocardial Mitochondria Protein after Two-Dimensional Electrophoresis
Wei Yiyong1, Li Ke2, Liu Yun3, Yu Shoujia5, Wang Haiying1, Yu Tian5*
1Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, China; 2Department of Anesthesiology, Stomatological Hospital Affiliated of Zunyi Medical University, Zunyi 563000, China; 3Research Center for Medicine & Biology, Zunyi Medical College, Zunyi 563000, China; 4Department of Critical Care Medicine, Zunyi First People’s Hospital, Zunyi 563000, China; 5Guizhou Key Laboratory of Fundamental Research on Anesthesiology and Organ Protection, Zunyi Medical College, Zunyi 563000, China
Abstract: There are the problems of the sensitivity and mass-spectrum compatibility for staining after twodimensional electrophoresis (2-DE) in proteomics. There are a lot of micro-proteins in mitochondria. These two properties are particularly important for the gel staining after 2-DE. Although the classic silver staining has a high sensitivity， the compatibility of mass spectrometry identification is low. Therefore， we optimized the silver staining for rat myocardial mitochondrial proteomics. We observed the image of gels and extracted some protein spots that were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) to verify the compatibility of mass spectrometry. The experimental results showed that the optimized method of staining has high sensitivity and good compatibility with mass spectrometry. The optimized silver staining is an ideal method in myocardial mitochondrial proteomics.
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