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Effects of Feeder Layer Type and Density on Culturing Rabbit (Leporidae) Primordial Germ Cells In Vitro
Wang Yadan, Hu Xiao, Ding Xuefen, Zhu Hanyu, Zhao Yuxi, Lü Haimiao, Peng Zhan, Wang Xinzhuang*
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
Abstract: The subject in the experiment was 14-18 d fetal rabbits. The primordial germ cells (PGCs) were isolated and cultured in vitro. After observing the morphological changes of PGCs and colony forming process， we used the AKP staining (alkaline phosphatase staining) and molecular biological methods to identify rabbit PGCs. Inoculate PGCs to mouse， rabbit embryo fibroblast feeder layer made of different density to determine the effects of the species and density on the culture of rabbit primordial germ cells in vitro. The results showed that rabbit PGCs were inoculated on feeder layers with a density of 6×104 made from homologous embryonic fibroblasts to obtain the largest number of colonies， the best colony morphology and slower differentiation.
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