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Wdr82 Regulates the Proliferation and Differentiation of mESCs
Zhang Wei1,2, Liu Lulu2, Hui Xinhui1, Wan Xiaoling2, Zhou Peipei2, Zhang Yan2*, Chen Hongxia1*
1School of Life Sciences, Shanghai University, Shanghai 200444, China; 2Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China
Abstract: Setd1a complex and Setd1b complex (Setd1a/b) are evolutionarily conserved histone-modifying enzymes that play critical roles in transcriptional activation by promoting methylation of histone H3 on Lysine 4 (H3K4). Setd1a/b complex includes Setd1a/b， Wdr82， Wdr5， Rbbp5， Ash2L， Dpy-30， Hcf-1. Wdr82 is a conserved non-catalytic subunit of Setd1a/b complex， however， the role of the Wdr82 during embryonic stem cells differentiation remains poorly understood. In this study， by moue embryonic stem cells (mESCs) cell culture and differentiation in vitro system， we found that Wdr82 mRNA level was decreased during mESCs differentiation. Wdr82 knockdown inhibited proliferation but not pluripotency of mESCs. Wdr82 knockdown slowed down embryoid body formation rate and contractile activity. Therefore， our results uncovered a vital role of Wdr82 in mESCs proliferation and differentiation.
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