Research Progress in Regeneration of Brassica Vegetables

Abstract: The establishment of regeneration system is important for functional gene analysis， preservation and propagation of important genetic material in Brassica vegetables. This paper summarized the factors that affected regeneration system of Brassica vegetables， such as genotype， phytohormone， AgNO3 and ascrobic acid， analyzed the differences of regeneration character under different factors among Brassica vegetables. In terms of genotype， Brassica rapa were more difficult regenerate than Brassica oleracea and Brassica juncea. For phytohormone， 2，4-D and NAA+6-BA were suitable for Brassica vegetables preculture and NAA+6-BA was fit for differentiation culture generally. Especially， different cultivar should be at different levels of hormones even at the same classification relationship. AgNO3 was the necessary exogenous additive for Brassica rapa and also could promote adventitious bud differentiation of Brassica oleracea and Brassica juncea. Low concentration of ascorbic aicd could promote the adventitious buds differentiation of Brassica rapa and the callus formation of Brassica oleracea. For future research， the molecular mechanism of induced regeneration and the difference of regeneration system in different kinds of Brassica vegetables should be emphasized.

CSTR: 32200.14.cjcb.2018.06.0026