The Regulation of Ct-OATP1B3 Gene on Migration， Invasion and Proliferation of Human Ovarian Cancer Cells

Abstract: Ct-OATP1B3 (cancer-type organic anion transporting polypeptide 1B3) is a molecules discovered in a variety of malignant tumor cells recently. It is a splicing isomer of solute carrier (SLC) superfamily member OATP1B3 which expressed in the liver， but its biological function in tumor cells is unclear so far. This study used immunohistochemical staining method to observe the expression of Ct-OATP1B3 in the tissues of normal ovary and ovarian cancer， and analyze the relationship between the expression positive rate and the clinicopathological characteristics of ovarian cancer. The expression of Ct-OATP1B3 in ovarian cancer cell lines SKOV3 and OVCAR3 were observed by Real-time PCR and Western blot. The localization of Ct-OATP1B3 in ovarian cancer cell lines SKOV3 and OVCAR3 were observed by cellular immunofluorescence staining. The effect of Ct-OATP1B3 on migration and invasion of ovarian cancer cells tested by Transwell. CCK-8 was employed to observe the effect of Ct-OATP1B3 on proliferation of ovarian cancer cells. The results showed that Ct-OATP1B3 was not expressed in normal ovarian tissues and cell line， and the expression of Ct-OATP1B3 was significantly increased in the ovarian cancer tissue， and the later in the clinical stage of ovarian cancer， the higher the positive rate of Ct- OATP1B3 was expressed. There is also a high expression of Ct-OATP1B3 in ovarian cancer cell line SKOV3 and OVCAR3， mainly in the cytoplasm. Our results showed that， although Ct-OATP1B3 had a moderate effect on the proliferation of ovarian cancer cells， its major role was to promote the abilities of ovarian cancer cell migration and invasion.

CSTR: 32200.14.cjcb.2018.04.0005