Home > Browse Issues > Vol.40 No.3
Effects of Arrb1 Knockout on the Development of Mice T Lymphocytes
Chen Yanhua, Jiang Guangjie, Guo Wei, Zhang Hang, Lü Wenqiong, Zou Lin*
Center for Clinical Molecular Medicine, the Children’s Hospital, Chongqing Medical University, Key Laboratory of Pediatrics in Chongqing, Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Scienceand Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China
Abstract: The purpose of this study was to identify the influence of β-arrestin1 (Arrb1) gene knockout on the development of mice T lymphocytes and lay the foundation for further study on the pathogenesis of T-cell acute lymphocytic leukemia (T-ALL) mechanism. The expression of Arrb1 was detected by q-PCR and Western blot respectively in Arrb1 knockout C57BL/6J mice. Cell suspensions prepared from the thymus， peripheral blood or lymph node of Arrb1 knockout and wild-type mice were detected by flow cytometry. Compared with wild-type mice， the Arrb1 knockout mice showed a higher proportion of CD4CD8 double negative (DN) cells， especially for cells in DN1 stage (P<0.05)， while it displayed a decreased proportion of cells in DN4 stage. Decreased proportion of cells CD4CD8 double positive (DP) cells in the thymus and CD4 positive T lymphoctes in the peripheral blood were detectable in Arrb1 knockout mice. Also， the proportion of CD4 positive T lymphocytes and the ratio of CD4/CD8 positive T lymphoctes in the lymph node was decreased (P<0.05). Overall， these findings indicated that sustained Arrb1 deficiency perturbs the development of T lymphocytes， which led to T lymphocytes arrest in DN stage， suggesting that Arrb1 might be important， particular in T-ALL progression.
CN
EN