Home > Browse Issues > Vol.40 No.2
Effects of GATA-1/2 on the Transcription of LUNA Gene in Human Monocytes Infected with HCMV
Guo Binhan1,2,3, Zhang Mengyi1,2,3, Liu Yanqing1,2,3, Lai Meimei1,2,3, Lu Yutian1,2,3, Wang Huiyan1,2, Zheng Xiaoqun1,2,3*
1The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2School of Laboratory Medical Science, Wenzhou Medical University, Wenzhou 325035, China; 3The Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou 325035, China
Abstract: This study investigated the relationship between transcription factor GATA-1/2 and human cytomegalovirus (HCMV) latency unique nuclear antigen (LUNA) gene using established HCMV latent and activated infection models. The protein level of GATA-1/2 were determined by Western blot. The binding of GATA- 1/2 with 5′ upstream sequence of LUNA gene were assessed by chromatin immnuoprecipitation (ChIP). Moreover， the level of LUNA mRNA was detected by RT-PCR after GATA-1/2 were interfered. The results showed that the expression of LUNA gene and GATA-1/2 in latent infection group were both higher than activated infection group，and the difference was statistically significant (P<0.01). The binding rate of transcription factor GATA-1/2 with 5′ terminal of promoter of LUNA gene in latent infection group was lower than that of activated infection group， and the difference was statistically significant (P<0.01). The expression of LUNA gene were both downregulated in HCMV latent and activated infection group after GATA-1/2 were interfered. Our results indicated that the transcription factor GATA-1/2 is involved in the presence of LUNA 5′ upstream promoter region in HCMV latent and activated infection， and participates in the process of HCMV latent and activated infection. Keywords
CN
EN