Multipotent of Monoclonal Epithelial Stem Cells Derived from Pancreatic Duct

Abstract: Monoclonal epithelial stem cells derived from pancreatic duct of an adult rat were in vitro induced to differentiate into pancreatic islets， nerve cells， adipose cells and osteoblasts. And pluripotency of these stem cells was studied. Monoclonal epithelia stem cells derived from pancreatic duct of an adult rat were proliferated. With different inducement medium， epithelial stem cells were respectively induced to differentiate into pancreatic islets， nerve cells， adipose cells and osteoblasts. Characteristics of differentiated cells were respectively detected with DTZ staining， glucose stimulation experiment， immune-fluorescence reaction， oil red staining， alizarin red staining or Vonkossa staining. The results showed that in inducement culture conditions， monoclonal epithelial stem cells derived from pancreatic duct of an adult rat differentiated into functional pancreatic islet-like tissue that was positive for DTZ staining， secreted insulin and C-peptide using glucose stimulation. Monoclonal epithelial stem cells were induced to differentiate into nerve-like cells which expressed neuron specific enolase， and were induced to differentiated into adipose-like cells that displayed accumulation of lipid vacuoles as detected by oil red O. Also stem cells were induced to differentiate into osteoblast-like cells that excretion formed mineralized nodules. Alizarin red or Vonkossa staining was positive. This indicated that the monoclonal epithelial stem cells derived from pancreatic duct of an adult rat were pluripotent stem cells that could differentiate into cells from three germ layers.

CSTR: 32200.14.cjcb.2018.01.0006