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Effects of SMC3 on the Proliferation， Migration and Sphere Formation of Lung Cancer A549 Cells
Yu Dongmei, Huang Ying, An Shu, Yang Yang, Liu Ying, Hao Qian, Xu Tianrui*, Guo Xiaoxi*
Cell Signaling Lab, Kunming University of Science and Technology, Kunming 650500, China
Abstract: In this study， the level of SMC3 (structural maintenance of chromosomes protein 3) protein was down-regulated by RNA interference， then we explore the effect of SMC3 on human lung cancer cell line A549. The level of SMC3 protein was determined by Western blot. The effect of SMC3 down-regulation on the proliferation and migration of A549 cells was detected by CCK-8 assay and Transwell assay. The effect of SMC3 down-regulation on the stemness of A549 cells was detected by sphere formation. The results showed that the pSUPER-SMC3 interference plasmid was successfully constructed， and the level of SMC3 protein was significantly decreased in A549 cells trasfected with the pSUPER-SMC3. Compared with the control group and the negative control group (pSUPER)， the cell proliferation and migration ability of the SMC3-knocked down group (pSUPERSMC3) decreased significantly (P<0.01)， and the number of sphere formation and the stemness decreased， indicating that SMC3 could promote the development and progression of lung cancer and might provide a new experimental basis for finding potential anti-lung cancer methods.
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