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A New Method to Isolate， Culture and Identify the Synovial Mesenchymal Stem Cells from the Patients with Temporomandibular Joint Osteoarthritis
Hu Chengchen1,2, Fang Zeqiang1, Feng Chi1, Pang Liang1,2, Ren Wei1,2, Qiu Lihua1*
1Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China; 2Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
Abstract: Our objective is to isolate and identify the character of synovial mesenchymal stem cells (SMSCs) derived from the rinse solution of patients with temporomandibular joint osteoarthritis. Synovial-derived mesenchymal stem cells were collected from rinse solution of the temporomandibular joint osteoarthritis patients， cultured in vitro and the cell growth curve and cell morphology were analyzed; cell surface markers were detected by immunofluorescence and stem cell markers were detected by flow cytometry. We also detected the osteogenic， adipogenic and chondrogenic differentiation ability and the secretion factor of the cell. The results showed that we could steadily obtain the SMSCs from rinse solution of the temporomandibular joint osteoarthritis patients by means of centrifugation and adherent culture. Our results showed the logarithmic growth in vitro， expression of the surface markers (CD73， CD90， CD105)， vimentin and alpha-SMA in cultured SMSCs. The SMSCs could also be induced to differentiate into osteogenic， chondrogenic and adipogenic lineages in vitro. More than 10 kinds of vascular growth factors such as FGF， angiopoietin， TGF-β can be detected from the cell secretion. These results indicated that SMSCs were obtained by minimally invasive method which provided a new resource of seed cell for the treatment of temporomandibular joint osteoarthritis.
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