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The Composition of BCR H-CDR3 Repertoires in Peripheral Blood and Spleen from 1， 3， 5 Months Old BALB/c Mice
Li Yuehong1#, Pan Yurong1#, Sun Suhong2#, Ma Long1, Shi Bin3, Ma Rui1, Yao Xinsheng1*
1Department of Immunology, Zunyi Medical College, Immunology Innovation Base of Postgraduate Education in Guizhou Province, Zunyi 563000, China; 2Department of Thyroid and Breast Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, China; 3Department of Medical Laboratory, Zunyi Medical College, Zunyi 563000, China
Abstract: B cell is the main component of adaptive immunity and the main cell group mediating specific humoral immune response. It mainly depends on the B cell receptor (BCR) to take part in antigen response. The variable region of B cell receptor is a complementary determinant region (CDR)， consisting of CDR1， CDR2， CDR3， among them， CDR3 has the most abundant diversity. This article through high-throughput sequencing analyzes BCR heavy chain (H)-CDR3 repertoire diversity， composition characteristics of CDR3 region gene or amino acids and bias usage in each subfamily gene of 1， 3， 5 months old mice， finds that BCR H-CDR3 repertoires of peripheral immune organs (spleen) and peripheral blood exist the corresponding dynamical change during the development of mice. The results of this study are helpful to explore the changes of BCR H-CDR3 repertoire during the development of organism and to provide basic data for the study of BCR H-CDR3 repertoire under pathological conditions.
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