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microRNA-378i Inhibits the Proliferation and Migration of Rhabdomyosarcoma Cells
Zhong Jiayun, Wang Jiao, Zhao Yunping, Chen Xiaoyan*
State Key Laboratory of Ophthalmology, Optometry and Vision Science, Wenzhou Medical University Affiliated Hospital of Ophthalmology and Optometry, Wenzhou 325027, China
Abstract: This study investigated the level of microRNA-378i (miR-378i) in rhabdomyosarcoma cells and striated muscle specimen and the influence of proliferation and migration caused by miR-378i in rhabdomyosarcoma cells. Quantitative RT-PCR (qRT-PCR) was performed to determine the level of miR-378i in human rhabdomyosarcoma cells and striated muscle specimens. Lipofectimine was used to transfect miR-378i mimics into rhabdomyosarcoma cells to upregulate the expression level of miR-378i. MTS， clone formation experiments， xCELLigence analysis and flow cytometry were performed to detect the proliferation， growth， migration and cell cycle of rhabdomyosarcoma cells， respectively. Western blot was used to identify the protein level of IGF1Rβ. The results showed that the level of miR-378i in rhabdomyosarcoma cells was significantly decreased compared with striated muscle specimens. Recovery expression level of miR-378i inhibited cell proliferation and migration， decreased the number of clones， and induced G1 or G2 phase arrest in rhabdomyosarcoma cells. It was also found that miR- 378i could deregulate protein level of IGF1Rβ. In conclusion， miR-378i inhibited the proliferation and migration of rhabdomyosarcoma cells.
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