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Knockdown of LDHA Inhibits Growth and EMT of Glioma Cells
Peng Guangquan, Han Xiu, Chen Xi, Tang Yu, Cheng Jie, Xiong Ermeng, Peng Wanxin, Gong Aihua*
Department of Basic Medical Sciences, School of Medicine, Jiangsu University, Zhenjiang 212013, China
Abstract: To investigate the effects of LDHA on the EMT (epithelial-to-mesenchymal transition) and growth of human glioma cells， we first detected LDHA expression in different glioma cell lines. Then， the sh-LDHA were transfected into the glioma cells U87MG. Colorimetric assay， flow cytometry， CCK-8 assay， colony formation assay， wound healing assay， Transwell experiment， q-PCR and Western blot were used to investigate the effect of LDHA activity， apoptosis， proliferation， migration and EMT induced by LDHA knockdown in U87MG cells. The results showed that sh-LDHA could significantly reduce LDHA expression both in mRNA and protein levels. At the same time， LDHA knockdown induced apoptosis in U87MG cells， while inhibited LDHA activity about 50%. The proliferation rate was lower than control group and migration of U87MG cells was much lower compared to sh-EGFP group (P<0.05). In addition， knockdown of LDHA induced EMT of U87MG cells. However， LDHA down-regulation did not exert an obvious effect on U251MG and SW1783 glioma cells. Taken together， our results suggested that LDHA played an important role in the EMT of glioma cells and affected growth of glioma cells.
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