Influence of Two Culture Configuration on the Post-Cryopreservation of Rat Hepatocytes

Abstract: In order to study the influence of cell culture configuration on cell function after cryopreservation， we propose to administer rat hepatocytes to two different microcarriers (entity microcarrier Cytodex， porous microcarrier Cytopore) and cryoperserved in –80 °C after being cultured in minute gravity and high density (cooling at 1 °C/min using 5% DMSO+0.4 mol/L sorbitol). After two weeks of cryopreservation， the cells and microcarrier materials were thawed. Through the cell metabolism index determination of glucose， albumin， urea and the observation of the cell growth and cell vitality to show the situation of the growth and metabolism cells cultured in two kinds of carriers. The result demonstrated that during the time of cell culture， the MTT value and metabolic index of Cytopore were about 1-1.5 times compared to Cytodex. Furthermore， there were no significant difference among the each indicators of Cytopore after cryopreservation， while the indicators of Cytodex were quite different. Hence， the cultivation of rat liver cells in carrier Cytopore with high density and cryopresreved is better for the growth， proliferation and metabolism of cells than that in the carrier Cytodex.

CSTR: 32200.14.cjcb.2017.08.0003