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The Study on the Changes of Biological Characteristics of BMSCs in the Tumor Microenvironment Simulated by C6 Glioma Treated by TPCA1
Yao Xiyou, Zhu Jing*, Tian Jie, Tan Bin, Li Xin
The Children’s Hospital Chongqing Medical University, Ministry of Education Key Laboratory of Developmental Disease in Childhood, Chongqing 400014, China
Abstract: This study investigated the way of avoiding BMSCs (bone mesenchymal stem cells) being malignant transformation in the tumor microenvironment simulated by C6 glioma to offer the reference for safety application. Experiment group was divided into three groups， the BMSCs was co-cultured indirectly with C6 glioma， treated by DMSO and TPCA1 (3-thiophenecarboxamide，2-[(aminocarbony 1) amino]-5-(4-fluoropheny 1) 1)， respectively. The changes of the cell morphology of each group were observed under microscope. The ability of migration and invasion was tested by Transwell. The mRNA levels of STAT3 (signal transducers and activators of transcription 3)， NF-κB (nuclear factor-κB)/p56 and C-myc were tested by Real-time PCR. The protein levels of P-STAT3， NF-κB/p56 and C-myc were measured by Wersten blot and immunofuorescence. The results shown that the cells treated by TPCA1 were more regular morphologically than untreated cells in experiment group. The ability of migration and invasion of the cells treated by TPCA1 group were lower than untreated group significantly. The mRNA and protein levels of STAT3， NF-κB and C-myc of the cells treated by TPCA1 were lower than untreated group significantly (P<0.05). The results confirmed that TPCA1 played a role in changing the biological characteristics of BMSCs in the tumor microenvironment simulated by C6 glioma.
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