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The Subcellular Localization of AnnAt2 in Arabidopsis
Chu Cuiping1, Ren Qiuping2, Kong Lanjing1, Wang Xiuling1*
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China; 2College of Agronomy, Liaocheng University, Liaocheng 252059, China
Abstract: Annexin is a highly conserved protein family binding to phospholipids in a calcium-dependent manner， while different annexins harbore various gene expression patterns and protein subcellular localizations. Eight annexins in Arabidopsis thaliana (AnnAt) have been identified. These annexins play important roles in growth， development and responses to stress. Annexin 2 in Arabidopsis thaliana (AnnAt2) is involved in root secretion and auxin-mediated geotropism growth of root cells. However， the molecular mechanism remains undefined. The subcellular localization of proteins is a crucial cue to explore their biological functions and molecular mechanisms. In the current study， the subcellular localization of AnnAt2 was investigated with fusion protein expression and colocalization with green fluorescent protein (GFP) of organelles or specific fluorescence dyes， respectively. Our results revealed that AnnAt2 was localized simultaneously in cytosol， nuclei， Golgi apparatus and endoplasmic reticulums. These data indicates that the translation and transportation of AnnAt2 are complex. AnnAt2 was colocalized with actin filaments marked by GFP in transgenic AnnAt2-mCherry Arabidopsis lines， suggesting that AnnAt2 might be involved in cell secretion via dynamic regulation of microfilaments and microfilament-mediated vesicles transport in Arabidopsis. These data provide the experimental evidence for further studies of protein translation， transport pathways and the functions of AnnAt2.
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