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The miR-197-3p Regulating Leptin Level in Schizophrenia Patients
Guo Tianhong1, Luo Binrui1, Wang Yuhan1, Peng Ying2, Bai Kezhen3, Huang Yuanshuai1*
1Department of Transfusion, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China; 2Department of Etiologies, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China; 3Department of Psychiatry, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China
Abstract: We analyzed the candidate microRNA (miRNA) levels in the serum of schizophrenia (SCZ)patients and controls， and explored the target mRNA of candidate miRNAs. Candidate miRNAs found by miRNA array were analyzed by Real-time PCR in a cohort including patients and healthy controls. The potential target mRNAs of miRNAs were analyzed by bio-informatics tools and studied by in vitro experiments. microRNA-197-3p (miR-197-3p) was found down-regulated in patients compared to controls by Real-time PCR. Leptin was found to be one of the targets of miR-197-3p by online bio-informatics tools. Leptin concentration was found up-regulated significantly in patients’ serum by ELISA. In vitro study illustrated that miR-197-3p targeted 3′ untranslated region (3′UTR) of leptin mRNA and reduced its expression. The pathway miR-197-3p regulating leptin may play an important role in schizophrenia pathogenesis.
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