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The Effects of Two Different PMA Induction on M1 and M2 Subtypes Related Genes Expression Profile in Differentiation and Polarization of THP-1 Derived Macrophages
Zhang Shujun*, Lei Qingsong, Li Lin, Qin Bo, Huang Wenxiang
Chongqing Key Laboratory of Infectious Diseases and Parasitic Diseases, Department of Infectious Diseases, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Abstract: THP-1 derived macrophages differentiated by phorbol 12-myristate 13-acetate (PMA) is the most common model in vitro for macrophages associated investigation. However， it is still controversial that how to use PMA in differentiation of THP-1 into macrophages. The present study examined the effects of two popular PMA induction on profile of M1 and M2 subtypes related genes by Real-time quantitative polymerase chain reaction (RT-qPCR). The results showed that high concentration induction could enhance the relative expression of interleukin-1 beta (IL-1β)， tumor necrosis factor-alpha (TNF-α)， inducible nitric oxide synthase (iNOS)， mannose receptor C-type 1 (MRC-1) and transforming growth factor-beta (TGF-β)， but suppress interleukin-10 (IL-10) in M0 macrophages compared with low concentration induction. Further， M0 macrophages differentiated by high concentration PMA induction have enhanced IL-1β but weakened response of IL-6 to interferon-gamma (IFN-γ)， while low concentration PMA induction enhanced response of both MRC-1 and DC-SIGN to interleukin-4 (IL-4). The results indicated that higher and lower concentration PMA inductions generated different phenotypes of THP-1 derived macrophages and polarized subtypes. Therefore， it is necessary to optimize PMA induction according to the goal and genes involved in specific experiments.
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