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Effects of Long Non-Coding RNA VLDLR on Proliferation and Migration of Human Pancreatic Cancer Cell
Sun Yaocheng1, Wang Dawei2, Xu Min2, Zhou Hailang2, Gong Aihua3, Chen Jixiang1*
1Department of General Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China; 2Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China; 3School of Medicine, Jiangsu University, Zhenjiang 212013, China
Abstract: The aim of this paper is to investigate the expression profile of long non-coding RNA VLDLR in pancreatic cancer tissues and 5 different cell lines and the effect of VLDLR on the proliferation and migration of pancreatic cancer cell. Quantitative reverse transcription PCR (qRT-PCR) was performed to detect the relative expression of VLDLR in 14 cases of pancreatic cancer tissues and corresponding para-carcinoma tissues and five cell lines. The proliferation rates were examined by CCK-8 and colony formation assays. The migration rates were examined by Transwell assays. The relative level of the protein was detected by Western blot. The relative level of VLDLR mRNA in 14 cases of pancreatic cancer tissues was 4.49±5.85 (P<0.05). The relative level of VLDLR mRNA were different in five pancreatic cancer cell lines. Compared with the control group， VLDLR mRNA level was decreased (t=32.43， 19.02， P<0.05) and the ability of proliferation， cell colony-forming and migration were significantly inhibited in experiment group (P<0.05). The protein levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 were reduced (P<0.05). These results indicated that the expression of VLDLR was up-regulated in pancreatic cancer. Knockdown of VLDLR could decrease the proliferation and migration of pancreatic cancer cell.
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