Home > Browse Issues > Vol.39 No.6
Effects of Astragalus polysaccharides on Osteogenic Differentiation of BMSCs Cultured in Low Oxygen Environment
Wu Zhiwei1,2*, Liu Dan1, Xue Na1, Liu Yongqi1,2, Su Yun1, Yan Chunlu1
1Basic Medical College, Gansu University of Chinese Medicine, Lanzhou 730000, China; 2Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with TCM Research in Gansu Colleges and Universities, Lanzhou 730000, China
Abstract: In the present study， the effects of Astragalus polysaccharides (APS) on osteogenic differentiation of bone mesenchymal stem cells (BMSCs) induced by osteogenic medium under hypoxic environment were analyzed. BMSCs were cultured by osteogenic induction medium containing 40 μg/mL Astragalus polysaccharides under the different oxygen concentration (3%， 6%， 10% and 20%) environments for 4 weeks. The osteocalcin particles existing in BMSCs-induced were detected by alizarin red staining. The levels of mRNA and protein of osteoblastic genes， runt-related transcription factor 2 (Runx2) and bone morphogenetic protein-2 (BMP-2)， in the BMSCs-induced were determined by Real-time PCR and Western blot， respectively. The results showed that， compared with the BMSCs-induced by pure osteogenic induction medium， more red orange osteocalcin particles were observed in BMSCs cultured in the medium containing Astragalus polysaccharide， and the mRNA and protein levels of Runx2 and BMP-2 were significantly increased， especially in 6% oxygen concentration (P<0.05). 40 μg/mL Astragalus polysaccharides could promote osteogenic differentiation of BMSCs-induced by osteogenic medium under hypoxic environment， and the role was related to oxygen concentration.
CN
EN