Apoptosis of Colon Cancer Cells Induced by Isorhamnetin and Its Mechanism

Abstract: To investigate the effect of isorhamnetin (ISO) on apoptosis of colon cancer and its mechanism， human colon cancer LOVO cells were treated with the different concentrations of isorhamnetin and growth inhibition was measured by MTT and PI assay. The morphologic change of adherent LOVO cell was tested by propidium iodide (PI) assay. The DNA ladder of LOVO cell was detected by DNA ladder assay. The relative levels of proteins were determined by Western blot. The results showed that isorhamnetin inhibited the proliferation of LOVO cells in dose- and time-dependent manner. And ISO also induced the apoptosis of LOVO cells. Further study revealed that isorhamnetin could up-regulate the level of apoptotic protein Bax and cleaved caspase-3， and down-regulate the level of the anti-apoptotic protein Bcl-2. It was also found that the epidermal growth factor receptor phosphorylation (pEGFR) was inhibited by isorhamnetin. The mechanism might relate to modulation of protein expressions associated cell apoptosis. These results provided a guide for laying down the chemotherapy for colon cancer， when isorhmnetin was used as anti-cancer agent.

CSTR: 32200.14.cjcb.2017.06.0002