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Observation on Guard Cell Microtubules from Hosta plantaginea by Immunofluorescence Method for Undergraduate Teaching
Xie Bingliang, Wang Yuhan, Zhang Lin, Wang Keyue, Lu Cunfu*
College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China
Abstract: Cytoskeleton observation is a significant experiment for undergraduate students majoring in biology. In the experimental teaching of cell biology， coomassie brilliant blue staining is the common method to observe plant cytoskeletons. However， it is not a good method to detect and distinguish different cytoskelons specifically. In present study， we used Allium cepa， Hosta plantaginea， and Buxus megistophylla to observe cell microtubules by immunofluorescence method. The results indicated that Hosta plantaginea， as an ornamental flower plant， is a suitable plant to observe clear fluorescent microtubule images in leaf guard cells. Microtubule depolymerization was detected in samples that treated at 4 °C or –20 °C， indicating that low temperature induced disintegration of the microtubule in guard cells. Therefore， Hosta plantaginea is a proper plant for observing microtubule by immunofluorescence microscopy for undergraduate teaching.
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