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Over-Expression of Chalcone Synthase of Safflower Increases Flavonoid Content in Arabidopsis thaliana
Liu Xiuming1,2, Lu Wanhang1, Li Jia2, Du Weihong2, Yao Na1, Dong Yuanyuan1, Meng Lulu3*, Li Haiyan1,2*
1Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China;
2College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;
3Science and Technology Department of Jilin Province, Changchun 130118, China
2College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;
3Science and Technology Department of Jilin Province, Changchun 130118, China
Abstract: The object of this study is to isolate the cDNA of CHS of safflower and overexpress it in Aropidopsis thaliana to vetify its function briefly. In this study， the full-length cDNA sequence of chalcone synthase (CHS) gene named CtCHS1 was cloned from flowers of Carthamus tinctorius L. (safflower) by RT-PCR and RACE techniques according to the sequences of transcriptome in safflower. The full-length cDNA of CtCHS1 was 1 360 bp and included a whole open reading frame of 1 113 bp， encoding a polypeptide with 370 amino acids. Subcellular localization prediction showed that CtCHS1 may locate in the cytoplasm. Safflower CHS gene is highly conservative according to the blasting and phylogenetic analysis. Real-time PCR results indicated that relative expression of CHS gene was highest in fade of JIHONGYOU var. and early flowering period of JIHONGYIHAO var.. Plant expression vector of CtCHS1 was constructed and transformed into A. thaliana genome. The T3 plants were obtained and the flavonoids content in transgenetic A. thaliana is higher than that in wild type. This results showed that over-expression of CtCHS1 could improve the flavonoids content and lay a foundation for the functional verification of CtCHS1 flavone content is determined in leaves，
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