Home > Browse Issues > Vol.38 No.8
The Mini-review of Techniques and Methods Used for Long Noncoding RNA Studies
Wang Hui, Zhang Lingling, Wang Bingrui, Ren Sirui, Gao Jie, Tong Jingyuan, Liu Jinhua*, Shi Lihong*
State Key Laboratory of Experimental Hematology, Institute of Hematology, Blood Disease Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
Abstract: Long noncoding RNA (lncRNA)， a novel class of noncoding RNA molecules longer than 200 nucleotides， are identified largely due to the rapid development of the next generation RNA sequencing technology.Recent studies reveal that lncRNAs play essential roles in a broad range of biological processes， including the embryonic development， lineage differentiation， genomic imprinting and disease pathology， etc. Given the necessary functions of lncRNAs， it is important to summary the techniques and methods currently used for lncRNA studies， which will shed more light on future lncRNA studies. Therefore， in this study we review the frequentlyused mothods for the identification， expression， function， as well as the regulatory mechanism of lncRNAs， and update their most recent development.
CN
EN