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The Effect of Different Fractions of Chicken Egg-White Extract on the Expression of the Pluripotency Factors in 293T Cells
Ruan Guangping, Yao Xiang, Liu Jufen, Wang Jinxiang, Pang Rongqing, Pan Xinghua*
The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command,Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming 650032, China
Abstract: This work was aim to investigate the effect of the chicken egg-white extract on the expression of pluripotent factors of 239T cells. We isolated chicken egg-white extract into 2 fractions， greater or less than 10 kDa fractions. And 2 different fractions were used with a final concentration of 50% to culture 293T cells for 5 days，respectively. And then， the levels of pluripotent factors in the 293T cells were detected by flow cytometry. The results showed that the chicken egg-white extract and its 2 fractions had the roles of increasing pluripotent factors in 293T cells. And the fractions of greater than 10 kDa had the most obvious effects with statistically significant (P<0.01). In conclusion， chicken egg-white extract and its fractions increased the expression of pluripotent factors in somatic cells. The chicken egg-white extract might have some substances to promote to reprogramme somatic cells.
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