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Autophagy Inhibition by Chloroquine Sensitizes Cervical Cancer SiHa Cells to CPT Treatment
Wang Xiaona1, Ren Yunqing2*, Wu Huiwen3, Zhao Jiawei4, You Hehua2, Song Binyu3, Yang Sai2, Ren Laifeng2*
1The First Clinical Medical College, Shanxi Medical university, Taiyuan 030001, China;
2Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200, China;
3Science and Technology Center, Fenyang College of Shanxi Medical University, Fenyang 032200, China;
4Department of Clinical Medicine, Xiamen University School of Medicine, Xiamen 361000, China
2Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200, China;
3Science and Technology Center, Fenyang College of Shanxi Medical University, Fenyang 032200, China;
4Department of Clinical Medicine, Xiamen University School of Medicine, Xiamen 361000, China
Abstract: The autophagy induction is one of the reasons for the resistance of tumor cells to chemotherapy drugs. In this study， the enhanced sensitivity of cervical cancer SiHa cells to camptothecin (CPT)-induced cell death by chloroquine (a lysosome inhibitor) was investigated. The cell viability was detected by MTT assay， meanwhile，apoptosis was observed by DAPI and TUNEL， autophagy related proteins and apoptosis proteins were analyzed by immunofluorescence (IF) staining and Western blot in SiHa cells after CPT treatment alone or combined with chloroquine. The results found that in SiHa cells with CPT treatment the autophagy related protein LC3 foci and microtubule-associated protein light chain 3II (LC3II) protein level was increased， but p62 foci and protein level was decreased. When autophagy was inhibited by chloroquine， the CPT-induced apoptosis was obviously enhanced， and caspase-9 was activated and PARP was cleaved， but full length caspase-2 was decreased. Taken together， these results indicated that the inhibition of autophagy by chloroquine could sensitize cervical cancer SiHa cells to CPT inducing cell apoptosis.
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