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Comparison of Vibratome and Frozen Sections of Brain Tissue in the Quality and Image of Confocal Laser Scanning Microscopy
Li Guiping1, Zhu Xiaona2, Yi Jing1, Hang Qin1*
1Department of Biochemistry and Molecular Cell Biology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China;
2Neuroscience Division, Department of Anatomy,Histology and Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
2Neuroscience Division, Department of Anatomy,Histology and Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
Abstract: We studied the quality of vibratome sections and frozen sections of brain tissues and evaluated the imaging quality of these two sections with laser scanning confocal microscope (LSCM)， respectively. Brain tissues of 6- to 8-week-old mice (CD1) were fixed by perfusion and then embedded in low-melting agarose， sliced on a vibratome， or embedded in OCT compound， sliced on a freezing microtome. The vibratome sections and the frozen sections were demonstrated by immunofluorescence staining and observed by confocal laser scanning microscopy. The results showed that compared with frozen sections， vibratome sections of brain tissues offered distinct advantages: easier embedding approaches， higher quality of brain tissues sections， better structural integrity of tissue sections， and superior image quality of confocal laser scanning microscopy.
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