Toosendanin Induces the Apoptosis of Human Lung Cancer A549 Cells

Abstract: To investigate the apoptosis-inducing effect of Toosendanin in human lung cancer A549 cells and its mechanisms， MTT assay was used to determine A549 cells activity. Light microscope， fluorescence microscope were used to observe the morphology change of apoptosis in A549 cells. The apoptosis， mitochondrial membrane potential (ΔΨm) and cell cycle distribution of A549 cells were assayed by flow cytometry. The mRNA and protein levels of Bax， Bcl-2， Fas， Cycs (Cytochrome C) and Caspase-3 were measured by Real-time RT-PCR and Western blot. The results showed that Toosendanin inhibited the growth and induced the apoptosis of A549 cells in a dose-dependent manner. The concentration for the treatment was optimized to 40 μmol/L after 48 h in this study and the inhibition rate was up to 46.73%±1.47% for the proliferation， the apoptotic rate could be up to 13.18%±0.41%. The level of ΔΨm decreased and caused a significant S arrest at the expense of G2 phase cell numbers.The results suggested that Toosendanin induced the apoptosis of lung cancer cells through up-regulation of Bax， Fas， Cycs and Caspase-3 and down-regulation of Bc1-2.

CSTR: 32200.14.cjcb.2015.08.0005