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Study of Downregulated LincRNA HOTAIR on Migration and Proliferation Inhibition of Gastric Cancer Cells
Chen Dengyu*, Xu Zhiben, Zheng Qingwei
Department of Pathogenic Biology, Bengbu Medical College, Anhui Key Laboratory of Infection and Immunity,Bengbu Medical College, Bengbu 233030, China
Abstract: This work was aim to study the effect of downregulating lincRNA HOTAIR expression on the migration and proliferation of BGC823 gastric cancer cells. The short hairpin RNA shHOTAIR was transfected into BGC823 gastric cancer cells， and the stably transfected cells were isolated by G418 selection. The lincRNA HOTAIR gene expression was detected by qPCR. The abilities of migration， invasion and proliferation of shHOTAIR transfected gastric cancer cells were investigated in the ways of wound healing and transwell invasion and MTT assays. The results showed that the abilities of migration， invasion and proliferation of the gastric cancer cells of low lincRNA HOTAIR expression were significantly decreased compared with the control group. Targeting lincRNA HOTAIR expression in gastric cancer cells with RNA interference could significantly reduce and inhibit the migration and proliferation abilities of gastric cancer cells. These data suggested that lincRNA HOTAIR could be one potential therapeutic target for human gastric caner treatment.
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