The Study of Noval Temperature-responsive Microcarriers for Non-invasive Harvest of Anchorage-dependent Cells

Abstract: Poly (N-isopropylacrylamide) (PNIPAAm) grafted microcarrier is a typical thermo-sensitive microcarrier due to the presence of hydrophilic amide chains and hydrophobic isopropyl chains in side-chains. However，the grafted amount of NIPAAm on cell culturing surface will greatly affect the adhering and detaching behavior of anchorage-dependent cells， which restricts its application. In our study， atom transfer radical polymerization (ATRP) method was used to graft different amounts of PNIPAAm onto the microcarrier surfaces. Then cell attaching， detaching and proliferation ability in stirred suspension culture were studied. The surface of thermo-microcarriers was characterized by SEM (scanning electron microscope)， AFM (atomic force microscope) and XPS (X-ray photoelectron spectroscopy) methods. The results revealed that when the feeding concentration was 200 mmol/L， the thermomicrocarriers showed the best cell detaching ability of about 60%， cell attaching ability of about 50% and steady proliferation ability in suspension culture. The results of SEM and AFM showed that thermo-microcarrier surfaces were much rougher than CMPS [chloromethylated poly (styrene)] microcarries， and XPS results showed that the content of nitrogen on microcarrier surface was increased significantly， which proved the successful grafting of PNIPAAm onto the surface of microcarriers. The thermo-responsive microcarrier exhibiting thermo-dependent cell adhesion and detachment will be an attractive candidate for the large-scale culturing of anchorage-dependent cells.

CSTR: 32200.14.cjcb.2015.07.0005