Home > Browse Issues > Vol.37 No.5
Cloning Methods for Engineering Recombinant Mammalian Cell Line
Xu Ruiyao1, Cai Jiexing2, Zhang Yubin1*
1School of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China; 2WuXi AppTec, Biologics & Bioprocess, Shanghai 200131, China
Abstract: Cloning plays an essential role in the development of recombinant cell line. Except cloning cylinders in early stage， limiting dilution cloning， semi-solid cultivation， flow cytometry and cell sorting are the most popular cloning methods in biopharmaceutical industry at present. This paper reviewed the principle and characteristic of these methods， introduced some new technologies which promote the development of cloning methods like single-cell imaging and automation and assessed the advantage and disadvantage of these methods. Some suggestions were given about how to make the best choice to facilitate researchers in this field.
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