Research Progress in Culture Condition of Mesenchymal Stem Cells during Long-term Subculture

Abstract: Mesenchymal stem cells (MSCs) are multipotent precursors derived from the stromal fraction of adult tissues. MSCs possess the capacity of self-renewal and multipotency to differentiate into adipocytes， osteoblasts and chondrocytes， which give rise to promising application in tissue engineering and cell therapy. The longterm subculture to harvest high numbers of MSCs is one of the most crucial factors for the application. However， optimal culture system has not been established so far. This paper reviewed the effect of basal medium， serum and growth factor of culture system on MSCs during the long-term subculture respectively， aiming to provide theoretical basis to establish optimal culture system for long-term subculture of MSCs.

CSTR: 32200.14.cjcb.2015.02.0016