The Preparation of a Novel Magnetic Nanoparticles Carrying TFPI-2 and Cisplatin and Its Evaluation of Inhibitory Effect on Nasopharyngeal Carcinoma In Vitro

Abstract: MNP-CDDP/TFPI-2 was prepared to construct a novel multifunctional magnetic nanocarrier loading tissue factor pathway inhibitor-2 (TFPI-2) and cisplatin (CDDP). We evaluated comprehensive inhibitory effect of this nanocomposites on nasopharyngeal carcinoma (NPC). MNP-CDDP/TFPI-2 was constructed with magnetic nanoparticles-cisplatin (MNP-CDDP) and (MPEG-PEI)TFPI-2. The CDDP concentration， morphology， particle size and zeta potential of nanocomposites were measured by OPDA (o-phenylenediamine) colourimetry， Fourier transform infrared spectrum (FT-IR)， transmission electron microscope (TEM) and laser particle detector， respectively. Flow cytometry was used to analyze the gene transfection efficiency of magnetic nanocomposites. TFPI-2 mRNA and protein expression levels were evaluated by RT-PCR and Western blot. Proliferation rate， cell apoptosis and invasion ability of CNE-2 cells cultured with MNP-CDDP/TFPI-2 were measured by CCK-8， flow cytometry and matrigel invasion test. The results showed that nanocomposites were successfully synthesized， with the average particle size 151.2 nm， Zeta potential +14.5 mV. OPDA showed that the CDDP concentration of nanocomposites was 120 μg/mL and the encapsulation efficiency of CDDP was 33.3%. The transfection efficiency of TFPI-2 in the nanocomposites was 22.7%±2.5%. The mRNA and protein expression levels of TFPI-2 in CNE-2 cells of MNP-CDDP/TFPI-2 group were significantly increased compared with those of the control group. The rate of growth inhibition and cell apoptosis were 34.8% and 42.3%， whereas that in MNP-CDDP group were 27.1% and 38.0%， in (MPEG-PEI)TFPI-2 group were 18.9% and 16.2%， respectively (P<0.05). Matrigel invasion assay showed that the number of transmembrane cells was 28±3 in MNP-CDDP/TFPI-2 group， which was lower than that in the control group (P<0.05). MNP-CDDP/TFPI-2 carrying cisplatin and TFPI-2 was successfully constructed and proved to be significantly inhibitory effect on the growth of CNE-2 cells.

CSTR: 32200.14.cjcb.2015.02.0007