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Cell Proliferation Effect Co-expression of hTERT and SV40 LT Gene on Bovine Mammary Gland Epithelial Cells In Vitro
He Xiaoying*, Ma Libing, Cheng Teng
School of Mathematics, Physics and Biological Engineering, Inner Mongolia University of Science and Technology, Baotou 104010, China
Abstract: Human telomerase reverse transcriptase (hTERT) and simian virus 40 large antigen (SV40 LT) can stimulate cell proliferation and extend cell life-span， however， they are low efficient. Therefore， it remains for further research to verify whether the two genes have co-expression patterns. In this study， the co-expression plasmid carrying hTERT and SV40 LT gene was constructed and transfected bovine mammary epithelial cells (bMGEs). The obtained positive cells could maintain growth through passage 30. Using RT-PCR and Western blot， the expressions of hTERT and SV40 LT were detected in positive cells. Furthermore， there were higher proliferation rates in the positive cells than those of control group by flow cytometer. The results showed that the co-expression of hTERT and SV40 LT might stimulate cell proliferation and extend cell life-span.
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