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The Effect of miR-4258-siRNA-Lentivirus on Epithelial-Messenchymal Transition in HK-2 Induced by Intermittent High Glucose
Cao Luoyuan, Lin Yinghua, Huang Baoying*, Fu Xianguo, Yang Jing, Lin Feng
The Affiliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China
Abstract: We constructd lentivirus-mediated miR-4258-siRNA and observed the effect of miR-4258-siRNALV on epithelial-messenchymal transition in HK-2 induced by intermittent high glucose. The HK-2 cells were divided into normal group， negative control and interference group. The expression of miR-4258 in HK-2 cells was detected by Real-time qPCR. The expressions of Fibronectin， E-cadherin and α-SMA were tested by Western blot. The results showed that the expression of miR-4258 was decreased significantly by lentivirus-mediated miR-4258- siRNA. The expression of Fibronectin and α-SMA were down-regulated markedly in interference group than those of negative control. Meanwhile， the expression of E-cadherin was up-regulated significantly in interference group than that of negative control. This study shows that miR-4258-siRNA can suppress epithelial-messenchymal transition in HK-2 cells induced by intermittent high glucose.
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