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Effects of LIN28 Gene on Proliferation and Migration of Human Pancreatic Cancer Cells PANC1 and SW1990
Bian Shihui1, Li Jie1, Gong Aihua2, Chen Hui1, Jiang Xiaomeng1, Zhang Youli1, Xu Min1*
1Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China; 2Jiangsu University, Zhenjiang 212000, China
Abstract: The paper aimed at investigating the effects of LIN28 expression in proliferation and migration of pancreatic cancer cells. The preliminary experiment results showed that the abilities of cell proliferation and migration of pancreatic cancer cells associated with the expression pattern of LIN28 gene. Then the specific shRNA against LIN28 and the expressing vector 3×Flag-LIN28 were transiently transfected into the pancreatic cancer cells PANC1 and SW1990， respectively. The results of RT-PCR and Western blot demonstrated that the expression of LIN28 was efficiently inhibited or enhanced at both mRNA and protein levels (P<0.05). CCK-8 and transwell assays revealed that LIN28 depletion decreased cell proliferation and migration rates on PANC1 cells (P<0.05)， and conversely LIN28 overexpression increased cell proliferation and migration rates on SW1990 (P<0.05). Taken together， our results suggested that the expression level of LIN28 related to the activities of proliferation and migration of pancreatic cancer cells. This study will be an experimental support for clarifying the regulation role of LIN28 in pancreatic cancer evolution.
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