Home > Browse Issues > Vol.36 No.11
Analysis of Differentiation Potency of Bovine Spermatogonial Stem Cells In Vitro
Luo Fenhua1, Wu Sachula1, Huang Junjiu2, Wu Yingji1*
1The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University,Hohhot 010021, China;
2State Key Laboratory of Biocontrol, School of Life Sciences and Key Laboratory of Reproductive Medicine of Guangdong Province, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510275, China
Abstract: Long-term culture of spermatogonial stem cells (SSCs) in vitro is the core technique of the spermatogonial stem cell manipulation in livestock. However， the method for long-term culture of livestock SSCs has not yet been established so far. One of the reasons is lack of a simple and effective approach for analysis of biological activity of the livestock SSCs. The purpose of this study was to establish a method for identification of the bovine SSCs (bSSCs) biological activity in vitro. Firstly， bSSCs were cultured. Then， bSSCs were induced with stem cell factor (SCF) for differentiation in vitro. During the induction， the cells were observed with microscope， and the differentiated cells were identified by immunofluorescence staining. The results from observitions showed that the morphology of the bSSCs was changed obviously after induction culture for 8 d; and the cell motion behavior revealed specific characteristic of spermatocytes. The results from immunofluorescence staining showed that the differentiated cells expressed Scp3， a marker gene of spermatocytes. The results above illustrate that the bSSCs cultured in vitro possess the potential for differentiation into spermatocytes. In this study， the analysis method for inducing differentiation of the bSSCs has been established， and it can be applied for identification of physiological potential of the bSSCs cultured in vitro. However， culture conditions of inducing differentiation in vitro can’t meet all of the requirements for bSSC meiosis， resulting in some phenomena not fitting completely with the characteristics of spermatocyte. The culture medium of inducing differentiation still needs improvement.
CN
EN